Calculator of Volatile Compound Concentrations in Standard Solutions prepared in the Accordance of new OIV Method

Guide WES Parameters Masses (A,B) Masses (0.1,0.5,1,2) Masses (QC) Peak areas in SS Concentrations RRF

Preparation of 40 % v/v Water Ethanol Solution (WES)

Measure 400 mL of absolute ethanol and pour it into a 1-liter volumetric flask. Bring the solution volume up to the 1-liter mark by adding distilled water and mix thoroughly.
Accurately determine the volumetric ethanol content in the WES (ABV_WES, %) and WES density using standard methods.

Preparation of Standard Solution A

Weight the empty 100 mL volumetric flask. Measure approximately 60.0 mL of the WES, pour it into the flask and weight it.
Pipette reagents into the flask one by one and weigh each reagent. Make up to volume with WES, mix thoroughly and weight it.
Determine the final mass of the flask with all reagents and WES added in the solution.

Enter the values:

Mass of empty flask for solution A, mg:

Mass of WES portion 1 added in solution A, m^WES1_A, mg:

Table SS-A. Purity Pⁱ_i and mass values of the reagents mⁱ_A added in solution A

i	Reagent i	Purity Pⁱ_i, %	Mass mⁱ_A, mg
1
2
3
4
5
6
7
8
9
10

Mass of WES portion 2 added in solution A, m^WES2_A, mg:

Final mass of flask with all compounds and WES added in solution A, mg:

Preparation of Standard Solution B

Weight the empty 100 mL volumetric flask. Measure approximately 60.0 mL of the WES, pour it into the flask and weight it.
Pipette the required amount of solution A into the flask and weigh. Make up to volume with WES and weight it.
Determine the final mass of the flask with all compounds and WES added in the solution.

Enter the values:

Mass of empty flask for solution B, mg:

Mass of WES portion 1 added in solution B, m^WES1_B, mg:

Table SS-B. Mass values m^A_B of the stock solutions A added in solution B

Mass m^A_B, mg

Mass of WES portion 2 added in solution B, m^WES2_B, mg:

Final mass of flask with all compounds and WES added in solution B, mg:

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Preparation of Standard Solution 0.1

Enter the values:

Mass of empty flask for solution 0.1, mg:

Mass of WES portion 1 added in solution 0.1, m^WES1_0.1, mg:

Table SS-0.1. Mass values m^A_0.1 of the stock solutions A added in solution 0.1

Mass m^A_0.1, mg

Mass of WES portion 2 added in solution 0.1, m^WES2_0.1, mg:

Final mass of flask with all compounds and WES added in solution 0.1, mg:

Preparation of Standard Solution 0.5

Enter the values:

Mass of empty flask for solution 0.5, mg:

Mass of WES portion 1 added in solution 0.5, m^WES1_0.5, mg:

Table SS-0.5. Mass values m^A_0.5 of the stock solutions A added in solution 0.5

Mass m^A_0.5, mg

Mass of WES portion 2 added in solution 0.5, m^WES2_0.5, mg:

Final mass of flask with all compounds and WES added in solution 0.5, mg:

Preparation of Standard Solution 1.0

Enter the values:

Mass of empty flask for solution 1.0, mg:

Mass of WES portion 1 added in solution 1.0, m^WES1_1.0, mg:

Table SS-1.0. Mass values m^A_1.0 of the stock solutions A added in solution 1.0

Mass m^A_1.0, mg

Mass of WES portion 2 added in solution 1.0, m^WES2_1.0, mg:

Final mass of flask with all compounds and WES added in solution 1.0, mg:

Preparation of Standard Solution 2.0

Enter the values:

Mass of empty flask for solution 2.0, mg:

Mass of WES portion 1 added in solution 2.0, m^WES1_2.0, mg:

Table SS-2.0. Mass values m^A_2.0 of the stock solutions A added in solution 2.0

Mass m^A_2.0, mg

Mass of WES portion 2 added in solution 2.0, m^WES2_2.0, mg:

Final mass of flask with all compounds and WES added in solution 2.0, mg:

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Preparation of Standard Solution QC

Enter the values:

Mass of empty flask for solution QC, mg:

Mass of WES portion 1 added in solution QC, m^WES1_QC, mg:

Table SS-QC. Mass values m^A_QC of the stock solutions A added in solution QC

Mass m^A_QC, mg

Mass of WES portion 2 added in solution QC, m^WES2_QC, mg:

Final mass of flask with all compounds and WES added in solution QC, mg:

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Data from chromatograms of solution B for the estimation RRF and WES for blank test

Acquire 2-3 chromatograms of standard solution B and WES. Measure peak areas for volatile compounds and ethanol and record the peak area values.

Table SS-B. Peak area values of compounds in calibration solution B

№	Compound	Pick area, units of area
№	Compound	1	2	3
1	congener №1
2	congener №2
3	congener №3
4	congener №4
5	congener №5
6	congener №6
7	congener №7
8	congener №8
9	congener №9
10	congener №10
11	ethanol

Table WES. Peak area values of compounds in WES

№	Compound	Pick area, units of area
№	Compound	1	2	3
1	congener №1
2	congener №2
3	congener №3
4	congener №4
5	congener №5
6	congener №6
7	congener №7
8	congener №8
9	congener №9
10	congener №10
11	ethanol

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Preparation of 40 % v/v Water Ethanol Solution (WES)

Preparation of Standard Solution A

Table SS-A. Purity Pii and mass values of the reagents miA added in solution A

Preparation of Standard Solution B

Table SS-B. Mass values mAB of the stock solutions A added in solution B

Preparation of Standard Solution 0.1

Table SS-0.1. Mass values mA0.1 of the stock solutions A added in solution 0.1

Preparation of Standard Solution 0.5

Table SS-0.5. Mass values mA0.5 of the stock solutions A added in solution 0.5

Preparation of Standard Solution 1.0

Table SS-1.0. Mass values mA1.0 of the stock solutions A added in solution 1.0

Preparation of Standard Solution 2.0

Table SS-2.0. Mass values mA2.0 of the stock solutions A added in solution 2.0

Preparation of Standard Solution QC

Table SS-QC. Mass values mAQC of the stock solutions A added in solution QC

Data from chromatograms of solution B for the estimation RRF and WES for blank test

Table SS-B. Peak area values ​​of compounds in calibration solution B

Table WES. Peak area values ​​of compounds in WES

Table SS-A. Purity Pⁱ_i and mass values of the reagents mⁱ_A added in solution A

Table SS-B. Mass values m^A_B of the stock solutions A added in solution B

Table SS-0.1. Mass values m^A_0.1 of the stock solutions A added in solution 0.1

Table SS-0.5. Mass values m^A_0.5 of the stock solutions A added in solution 0.5

Table SS-1.0. Mass values m^A_1.0 of the stock solutions A added in solution 1.0

Table SS-2.0. Mass values m^A_2.0 of the stock solutions A added in solution 2.0

Table SS-QC. Mass values m^A_QC of the stock solutions A added in solution QC

Table SS-B. Peak area values of compounds in calibration solution B

Table WES. Peak area values of compounds in WES