Calculator of Volatile Compound Concentrations in Standard Solutions prepared in the Accordance of OIV Methods

Guide WES Parameters Masses (A,B,C) Masses (0,0.1,0.5,1,2) Masses (D,E,QC,BT) Peak areas in SS Concentrations RRF

Preparation of 40 % v/v Water Ethanol Solution (WES)

Measure 400 mL of absolute ethanol and pour it into a 1-liter volumetric flask. Bring the solution volume up to the 1-liter mark by adding distilled water and mix thoroughly.
Accurately determine the volumetric ethanol content in the WES (ABV_WES, %) and WES density using standard methods.

Preparation of Standard Solution A

Weight the empty 100 mL volumetric flask. Measure approximately 60.0 mL of the WES, pour it into the flask and weight it.
Pipette reagents into the flask one by one and weigh each reagent. Make up to volume with WES, mix thoroughly and weight it.
Determine the final mass of the flask with all reagents and WES added in the solution.

Enter the values:

Mass of empty flask for solution A, mg:

Mass of WES portion 1 added in solution A, m^WES1_A, mg:

Table SS-A. Purity Pⁱ_i and mass values of the reagents mⁱ_A added in solution A

i	Reagent i	Purity Pⁱ_i, %	Mass mⁱ_A, mg
1
2
3
4
5
6
7
8
9
10

Mass of WES portion 2 added in solution A, m^WES2_A, mg:

Final mass of flask with all compounds and WES added in solution A, mg:

Preparation of Standard Solution B

Weight the empty 100 mL volumetric flask. Measure approximately 60.0 mL of the WES, pour it into the flask and weight it.
Pipette IS reagent into the flask and weigh it. Make up to volume with WES, mix thoroughly and weight it.
Determine the final mass of the flask with IS reagent and WES added in the solution.

Enter the values:

Mass of empty flask for solution B, mg:

Mass of WES portion 1 added in solution B, m^WES1_B, mg:

Table SS-B. Purity P^IS_IS and mass value of IS m^IS_B added in solution B

Reagent IS name	Purity P^IS_IS, %	Mass m^IS_B, mg

Mass of WES portion 2 added in solution B, m^WES2_B, mg:

Final mass of flask with all compounds and WES added in solution B, mg:

Preparation of Standard Solution C

Weight the empty 100 mL volumetric flask. Measure approximately 60.0 mL of the WES, pour it into the flask and weight it.
Pipette the required amount of solution A into the flask and 1 mL of solution B and weigh each compound. Make up to volume with WES and weight it.
Determine the final mass of the flask with all compounds and WES added in the solution.

Enter the values:

Mass of empty flask for solution C, mg:

Mass of WES portion 1 added in solution C, m^WES1_C, mg:

Table SS-C. Mass values m^A_C and m^B_C of the stock solutions A and B added in solution C

Mass m^A_C, mg	Mass m^B_C, mg

Mass of WES portion 2 added in solution C, m^WES2_C, mg:

Final mass of flask with all compounds and WES added in solution C, mg:

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Preparation of Standard Solution 0

Enter the values:

Mass of empty flask for solution 0, mg:

Mass of WES portion 1 added in solution 0, m^WES1₀, mg:

Table SS-0. Mass values m^A₀ and m^B₀ of the stock solutions A and B added in solution 0

Mass m^A₀, mg	Mass m^B₀, mg

Mass of WES portion 2 added in solution 0, m^WES2₀, mg:

Final mass of flask with all compounds and WES added in solution 0, mg:

Preparation of Standard Solution 0.1

Enter the values:

Mass of empty flask for solution 0.1, mg:

Mass of WES portion 1 added in solution 0.1, m^WES1_0.1, mg:

Table SS-0.1. Mass values m^A_0.1 and m^B_0.1 of the stock solutions A and B added in solution 0.1

Mass m^A_0.1, mg	Mass m^B_0.1, mg

Mass of WES portion 2 added in solution 0.1, m^WES2_0.1, mg:

Final mass of flask with all compounds and WES added in solution 0.1, mg:

Preparation of Standard Solution 0.5

Enter the values:

Mass of empty flask for solution 0.5, mg:

Mass of WES portion 1 added in solution 0.5, m^WES1_0.5, mg:

Table SS-0.5. Mass values m^A_0.5 and m^B_0.5 of the stock solutions A and B added in solution 0.5

Mass m^A_0.5, mg	Mass m^B_0.5, mg

Mass of WES portion 2 added in solution 0.5, m^WES2_0.5, mg:

Final mass of flask with all compounds and WES added in solution 0.5, mg:

Preparation of Standard Solution 1.0

Enter the values:

Mass of empty flask for solution 1.0, mg:

Mass of WES portion 1 added in solution 1.0, m^WES1_1.0, mg:

Table SS-1.0. Mass values m^A_1.0 and m^B_1.0 of the stock solutions A and B added in solution 1.0

Mass m^A_1.0, mg	Mass m^B_1.0, mg

Mass of WES portion 2 added in solution 1.0, m^WES2_1.0, mg:

Final mass of flask with all compounds and WES added in solution 1.0, mg:

Preparation of Standard Solution 2.0

Enter the values:

Mass of empty flask for solution 2.0, mg:

Mass of WES portion 1 added in solution 2.0, m^WES1_2.0, mg:

Table SS-2.0. Mass values m^A_2.0 and m^B_2.0 of the stock solutions A and B added in solution 2.0

Mass m^A_2.0, mg	Mass m^B_2.0, mg

Mass of WES portion 2 added in solution 2.0, m^WES2_2.0, mg:

Final mass of flask with all compounds and WES added in solution 2.0, mg:

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Preparation of Standard Solution D

Weight the empty 100 mL volumetric flask. Measure approximately 60.0 mL of the WES, pour it into the flask and weight it.
Pipette the required amount of stock SS into the flask and weigh it. Make up to volume with WES and weight the second portion of WES.
Determine the final mass of the flask.

Enter the values:

Mass of empty flask for solution D, mg:

Mass of WES portion 1 added in solution D, m^WES1_D, mg:

Mass of SS-A added in solution D, m^A_D, mg:

Mass of WES portion 2 added in solution D, m^WES2_D, mg:

Final mass of flask with all compounds and WES added in solution D, mg:

Preparation of Standard Solution E

Enter the values:

Mass of empty flask for solution E, mg:

Mass of WES portion 1 added in solution E, m^WES1_E, mg:

Mass of SS-B added in solution E, m^B_E, mg:

Mass of WES portion 2 added in solution E, m^WES2_E, mg:

Final mass of flask with all compounds and WES added in solution E, mg:

Preparation of QC Solution

Weight the empty vessel (flask). Pipette 9 mL of solution D into the vessel and 1 mL of solution E and weigh each compound.
Thoroughly mix the contents of the vessel and determine the final mass of the flask.

Enter the values:

Mass of empty flask for solution QC, mg:

Mass of SS-D added in solution QC, m^D_QC, mg:

Mass of SS-E added in solution QC, m^E_QC, mg:

Final mass of flask with all compounds and WES added in solution QC, mg:

Preparation of BT Solution

Weight the empty vessel (flask). Pipette 9 mL of the WES into the vessel and 1 mL of solution E and weigh each compound.
Thoroughly mix the contents of the vessel and determine the final mass of the flask.

Enter the values:

Mass of empty flask for solution BT, mg:

Mass of WES portion added in solution BT, m^WES_BT, mg:

Mass of SS-E added in solution BT, m^E_BT, mg:

Final mass of flask with all compounds and WES added in solution BT, mg:

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Data from chromatograms of solutions C for the estimation RRF and BT for blank test

Acquire 2-3 chromatograms of standard solutions C and BT. Measure peak areas for volatile compounds and ethanol and record the peak area values.

Table SS-C. Peak area values of compounds in calibration solution C

№	Compound	Pick area, units of area
№	Compound	1	2	3
1	congener №1
2	congener №2
3	congener №3
4	congener №4
5	congener №5
6	congener №6
7	congener №7
8	congener №8
9	congener №9
10	congener №10
11	ethanol
12	traditional IS

Table SS-BT. Peak area values of compounds in blank test solution

№	Compound	Pick area, units of area
№	Compound	1	2	3
1	congener №1
2	congener №2
3	congener №3
4	congener №4
5	congener №5
6	congener №6
7	congener №7
8	congener №8
9	congener №9
10	congener №10
11	ethanol
12	traditional IS

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Preparation of 40 % v/v Water Ethanol Solution (WES)

Preparation of Standard Solution A

Table SS-A. Purity Pii and mass values of the reagents miA added in solution A

Preparation of Standard Solution B

Table SS-B. Purity PISIS and mass value of IS mISB added in solution B

Preparation of Standard Solution C

Table SS-C. Mass values mAC and mBC of the stock solutions A and B added in solution C

Preparation of Standard Solution 0

Table SS-0. Mass values mA0 and mB0 of the stock solutions A and B added in solution 0

Preparation of Standard Solution 0.1

Table SS-0.1. Mass values mA0.1 and mB0.1 of the stock solutions A and B added in solution 0.1

Preparation of Standard Solution 0.5

Table SS-0.5. Mass values mA0.5 and mB0.5 of the stock solutions A and B added in solution 0.5

Preparation of Standard Solution 1.0

Table SS-1.0. Mass values mA1.0 and mB1.0 of the stock solutions A and B added in solution 1.0

Preparation of Standard Solution 2.0

Table SS-2.0. Mass values mA2.0 and mB2.0 of the stock solutions A and B added in solution 2.0

Preparation of Standard Solution D

Preparation of Standard Solution E

Preparation of QC Solution

Preparation of BT Solution

Data from chromatograms of solutions C for the estimation RRF and BT for blank test

Table SS-C. Peak area values ​​of compounds in calibration solution C

Table SS-BT. Peak area values ​​of compounds in blank test solution

Table SS-A. Purity Pⁱ_i and mass values of the reagents mⁱ_A added in solution A

Table SS-B. Purity P^IS_IS and mass value of IS m^IS_B added in solution B

Table SS-C. Mass values m^A_C and m^B_C of the stock solutions A and B added in solution C

Table SS-0. Mass values m^A₀ and m^B₀ of the stock solutions A and B added in solution 0

Table SS-0.1. Mass values m^A_0.1 and m^B_0.1 of the stock solutions A and B added in solution 0.1

Table SS-0.5. Mass values m^A_0.5 and m^B_0.5 of the stock solutions A and B added in solution 0.5

Table SS-1.0. Mass values m^A_1.0 and m^B_1.0 of the stock solutions A and B added in solution 1.0

Table SS-2.0. Mass values m^A_2.0 and m^B_2.0 of the stock solutions A and B added in solution 2.0

Table SS-C. Peak area values of compounds in calibration solution C

Table SS-BT. Peak area values of compounds in blank test solution