Methods EIS and TIS are suitable for the gas chromatography determination of the following congeners in spirit drinks and wine: acetaldehyde, methyl acetate, ethyl acetate, acetal, methanol, 2-propanol, 2-butanol, n-propanol, 2-methyl-1-propanol, n-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol with analyte content ranging from 5 to 1500 g per 100 L anhydrous ethanol (g/100 L AA).

The new EIS method uses ethanol as an internal standard: ethyl alcohol is present in all samples and serves as a reference substance.

In conventional IS methods, traditional internal standard (TIS) is added to all samples. The TIS examples are: 3-pentanol, 1-pentanol, 4-methyl-1-pentanol, methylnonanoate, tert-amyl alcohol, n-amyl acetate, n-butyl acetate, n-pentyl acetate, 2-ethylbutyric acid, tetradecanol.

Use only reagents of a purity (P, %) greater than 97.0 %, with certificate of purity.

To work according to the both EIS and TIS methods, the following standard solutions (SS) of volatile compounds and internal standard (IS) are prepared:

• 40 % v/v ethanol solution in water (WES),
• stock SS - A, B, D, E,
• SS-C for establishing the calibration coefficients RRF,
• SS for the control of the FID response linearity - 0, 0.1, 0.5, 1.0 (or 5), 2.0 (or 30),
• SS-QC for the checking of the calibration quality,
• WES solution containing IS for blank test (BT).

To work according to EIS method only, the following standard solutions (SS) of volatile compounds are prepared:

• 40 % v/v ethanol solution in water (WES),
• stock SS-A,
• SS-B for establishing the calibration coefficients RRF,
• SS for the control of the FID response linearity - 0.1, 0.5, 1.0 (or 5), 2.0 (or 30),
• SS-QC for the checking of the calibration quality,

There are three version of "on-line calculator" program: to simultaneously work with in TIS and EIS methods, to work by only EIS method at concentration up to 150 g/100L AA and to work by only EIS method at concentration up to 1500 g/100L AA.

The calculator has a "Main Menu" for navigation. To transfer user-entered data into the calculator, press the button "Press to Submit Data".

"Main Menu" navigation panel contains the link (versions 1, 2, 3) to full pdf-file "GUIDE Calculation of Compound Concentrations in Standard Solutions and Calibration Factors during preparation the solutions in the accordance of the new OIV method OENO-SCMA 24-756 Et5_2026, using contained ethanol as a reference standard, (EIS method) and the OIV-MA-BS-14 method (TIS method)".

Example of downloaded data file *.txt:

The data fields in *.txt files are separated by semicolons, so, select the semicolon as delimiter to open these files in Excel.

Example of the file with results in Excel:

Example of saved file with premier data and results as *.pdf

If your browser do not support pdf, then the example of data file can be download
Example.pdf

Online calculator versions:
Calculator EIS and TIS methods
Calculator EIS method up to 150 g/100L AA
Calculator EIS method up to 1500 g/100L AA